Restricted Water Intake Adversely Affects Rat Vocal Fold Biology


The Laryngoscope
 
Abstract
 
Objectives

A holistic understanding of the many ways that systemic dehydration affects vocal fold biology is still evolving. There are also myriad physiologically relevant methodologies to induce systemic dehydration. To untangle the effects of systemic dehydration on vocal fold biology, we need to utilize realistic, clinically translatable paradigms of systemic dehydration in lab animals. Restricted access to water accommodates clinical translation. We investigated whether systemic dehydration via reduced water intake would negatively affect vocal fold biology.

Study Design

Prospective, in vivo study design.

Methods

Male Sprague Dawley rats (N = 13) were provided 4 mL/100 g of water/day for 5 days, whereas male control rats (N = 8) were given ad lib access to water. Following euthanasia, tissues were processed for histological staining, gene expression, and protein assays.

Results

Renin gene expression level in kidneys increased significantly (P ≤ .05), validating dehydration. Dehydration induced by restricted water access downregulated the gene expression of interleukin‐1α and desmoglein‐1 (P ≤ .05). Hyaluronidase‐2 gene expression increased after dehydration (P ≤ .05). The protein level of desmoglein‐1 decreased after dehydration (P ≤ .05). Histological analyses suggested decreased hyaluronan (P ≤ .05) in the water‐restricted rat vocal fold.

Conclusion

Reduced daily water intake for just 5 days impairs vocal fold biology by disrupting inflammatory cytokine release, reducing plasma membrane integrity, and disrupting the hyaluronan network. This is the first study investigating the dehydrating effects of restricted water intake on vocal fold tissue in an in vivo model.

 

 

 

 

 

 

 

 

 

 

 

Please reload

Recent Posts

Please reload

Archive

Please reload

Tags

Please reload

Address

University of Colorado - Boulder

1111 Engineering Drive
UCB 427
Boulder, Colorado 80309-0427

Contact

Phone: 765 496-1768

© 2020 by MEML